A histochemical study of the Golgi apparatus or 'acroblast' of the spermatid of the snail, Helix aspersa
نویسنده
چکیده
The acroblast of Helix spermatids is a duplex structure: it consists of an 'externum' (which appears dark by positive phase-contrast microscopy and becomes impregnated with osmium) enclosing a region called the 'internum'. Histochemical studies show the externum to be composed principally of phospholipid and to contain glycolipid, alkaline phosphatase, and an enzyme which splits thiamine pyrophosphate. The internum contains neutral mucopolysaccharide. Ribonucleic acid and acid phosphatase are not present in the acroblast. These histochemical results suggest a homology between the invertebrate acroblast and the Golgi apparatus in vertebrate cells.
منابع مشابه
Localization of Thiamine Pyrophosphatase Activity in the Golgi Apparatus of a Mollusc, Helix Aspersa
Spermatids of the snail Helix aspersa were studied after fixation in buffered osmium tetroxide and after applying Novikoff and Goldfischer's method (15) for demonstrating thiamine pyrophosphatase (TPPase) activity both with the light and the electron microscope. The appearance of cells in the light microscope after localizing the enzyme is very similar to the appearance after the application of...
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